이승구 교수님: Engineered Methylococcus capsulatus Bath for efficient methan…
페이지 정보
작성자 최고관리자
조회 1,700회 작성일 2024-03-07 15:35
본문
Engineered Methylococcus capsulatus Bath for efficient methane conversion to isoprene
https://doi.org/10.1016/j.biortech.2023.130098
abstract: Isoprene has numerous industrial applications, including rubber polymer and potential biofuel. Microbial methane-based isoprene production could be a cost-effective and environmentally benign process, owing to a reduced carbon footprint and economical utilization of methane. In this study, Methylococcus capsulatus Bath was engineered to produce isoprene from methane by introducing the exogenous mevalonate (MVA) pathway. Overexpression of MVA pathway enzymes and isoprene synthase from Populus trichocarpa under the control of a phenol-inducible promoter substantially improved isoprene production. M. capsulatus Bath was further engineered using a CRISPR-base editor to disrupt the expression of soluble methane monooxygenase (sMMO), which oxidizes isoprene to cause toxicity. Additionally, optimization of the metabolic flux in the MVA pathway and culture conditions increased isoprene production to 228.1 mg/L, the highest known titer for methanotroph-based isoprene production. The developed methanotroph could facilitate the efficient conversion of methane to isoprene, resulting in the sustainable production of value-added chemicals.
- 이전글이승구 교수님 : Unlocking synergies: Harnessing the potential of biological methane sequestration through metabolic coupling between Methylomicrobium alcaliphilum 20Z and Chlorella sp. HS2 24.03.29
- 다음글성봉현 교수님: Efficient valorization of food waste oils to renewable biodiesel by a Candida antarctica lipase B mutant that catalyzes the ester synthesis reaction in the presence of water 24.03.07