연구성과

이상엽 교수님: Targeted and high-throughput gene knockdown in diverse bacter…

페이지 정보

profile_image

작성자 최고관리자

조회 1,096회 작성일 2024-03-07 14:23

본문

Targeted and high-throughput gene knockdown in diverse bacteria using synthetic sRNAs 


https://doi.org/10.1038/s41467-023-38119-y 


abstract: Synthetic sRNAs allow knockdown of target genes at translational level, but have been restricted to a limited number of bacteria. Here, we report the development of a broad-host-range synthetic sRNA (BHR-sRNA) platform employing the RoxS scaffold and the Hfq chaperone from Bacillus subtilis. BHR-sRNA is tested in 16 bacterial species including commensal, probiotic, pathogenic, and industrial bacteria, with >50% of target gene knockdown achieved in 12 bacterial species. For medical applications, virulence factors in Staphylococcus epidermidis and Klebsiella pneumoniae are knocked down to mitigate their virulence-associated phenotypes. For metabolic engineering applications, high performance Corynebacterium glutamicum strains capable of producing valerolactam (bulk chemical) and methyl anthranilate (fine chemical) are developed by combinatorial knockdown of target genes. A genome-scale sRNA library covering 2959 C. glutamicum genes is constructed for high-throughput colorimetric screening of indigoidine (natural colorant) overproducers. The BHR-sRNA platform will expedite engineering of diverse bacteria of both industrial and medical interest.


41c0ddd90614d636c0db1bc2d2235d49_1709788977_5897.JPG