성봉현 교수님: Development of novel recombinant peroxidase secretion system …
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조회 1,006회 작성일 2024-03-07 13:38
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Development of novel recombinant peroxidase secretion system from Pseudomonas putida for lignin valorisation
https://www.sciencedirect.com/science/article/pii/S0960852423012075?via%3Dihub
abstract: Pseudomonas putida is a promising strain for lignin valorisation. However, there is a dearth of stable and efficient systems for secreting enzymes to enhance the process. Therefore,
a novel secretion system for recombinant lignin–depolymerising peroxidase was developed. By adopting a flagellar type III secretion system, P. putida KT-M2, a secretory host strain, was constructed and an optimal secretion signal fusion partner was identified. Application of the dye-decolourising peroxidase of P. putida to this system resulted in efficient oxidation activity of the cell-free supernatant against various chemicals, including lignin model compounds. This peroxidase-secreting strain was examined to confirm its lignin utilisation capability, resulting in the efficient assimilation of various lignin substrates with 2.6-fold higher growth than that of the wild-type strain after 72 h of cultivation. Finally, this novel system will lead efficient bacterial lignin breakdown and utilization through enzyme secretion, paving the way for sustainable lignin-consolidated bioprocessing.
