My research aims to use synthetic biology tools to engineer microbes for the purpose of developing live therapeutics. In order to achieve this goal, I have developed synthetic biology toolkits specifically for the human microbiome. The focus of my ongoing research activities are twofold: Firstly, I aim to develop molecular and genetic tools for gut microbes. Gut microbes are crucial for human health, and their beneficial functions at the molecular level have been revealed. However, it is challenging to identify beneficial microbes from the human microbiome, particularly for disease treatment. To address this challenge, I will use the design-build-test-learn cycle of synthetic biology to create new tools for gut microbes. Secondly, I aim to create a biofoundry workflow for an automated design-build-test-learn cycle. While genetic circuits have been developed and utilized for developing disease-sensing microbes, more complex functions are necessary for therapeutic microbes. Therefore, CRISPR technologies will be used in conjunction with genetic circuits to create complex functions, such as logic gates. This process will be streamlined through automation using the biofoundry workflow.